microscope nikon eclipse te 2000u  (Nikon)

Journal: International Journal of Molecular Sciences

Article Title: Differential Regulation of Hemichannels and Gap Junction Channels by RhoA GTPase and Actin Cytoskeleton: A Comparative Analysis of Cx43 and Cx26

doi: 10.3390/ijms25137246

Figure Lengend Snippet: TIRFM configuration to study HCs transport to appositional and non-appositional plasma membrane zones. A representative cartoon showing two cells in juxtaposition and all the components involved in the data acquisition. TIRF microscopy configuration utilizes a critical angle, which creates a 100–200 nm-deep evanescent wave between the glass coverslip surface and cells to excite fluorophores located in the vicinity of the plasma membrane. Analysis was performed in the appositional and non-appositional zones ( a ). An example of how TIFR configuration is achieved under the microscopy is shown ( b ). Tagged connexins were illuminated in epifluorescence (left panel). Then, the surface reflective interference contrast (SRIC) filter ensures an appropriate field with cells attached to the glass (middle panel). Finally, it is switched to TIRF microscopy configuration for the data acquisition (right panel) ( b ).

Article Snippet: Images were acquired using a Nikon Eclipse TE-2000U epifluorescence inverted microscope.

Techniques: Clinical Proteomics, Membrane, Microscopy